Figure 6. Late BDNF changes after HI.

Higher BDNF levels observed in the forebrain of the hemisphere ipsilateral to the injury correlates with worse memory outcomes regardless of treatment. Grouped BDNF data for ipsilateral (A), contralateral (B) and ipsilateral/ contralateral (I/C) ratio (C) and sex-stratified I/C ratio (D) are represented as box and whiskers plots, where boxes represent the interquartile range (IQR) limited by the 25^th and 75^th percentile (lower and upper limit, respectively), line inside the box indicates the median and whiskers extend to 1.5 times the IQR. Outliers are not represented. White boxes represent shams, black boxes represent NT mice, and grey boxes represent TH mice. *, p<0.05 vs. sham, one-way ANOVA (grouped or stratified by sex) with post-hoc analysis using Tamhane (n=14 per group for panels A, B and C, and n = 7 per group/ per sex for panel D). Subject-by subject correlation between BDNF levels in ipsilateral hemisphere (E, H), contralateral hemisphere (F, I) and I/C ratio (G, J) vs. % entries to novel arm (Y-maze phase 2, group data) and EP score (OLT, male mice). Analysis by Spearman Rho. r, correlation coefficient. Significance determined by p<0.05. Representative immunoblots for pTrkB, TrkB and loading control with β-actin (n=5/group) shows no significant receptor activation despite BDNF increase in hemisphere ipsilateral to injury.