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Conversion of thiocarbamates 7 and 12 into the respective corresponding free thiols: (a) compound 7 into 14 and (b) compound 12 into 15. Time course of hydrolysis in 10 mM Hepes, pH 7.4 (10% methanol), at 22.5 °C was monitored by LC-MS, showing conversion into corresponding thiol and to minor extent into another compound which is most likely the disulfide oxidation product.79
