Figure 9.

PMN depletion via anti-Ly6G increased the proportion of monocytes but did not affect the absolute number of blood monocytes at 1 dpi and up to 14 dpi following hCNS-SCns transplantation. A–D, hCNS-SCns + anti-Ly6G treatment (B) had increased blood monocyte proportion at 1 dpi compared with hCNS-SCns + IgG2a treatment (A), demonstrating an increase in the percentage of monocytes in blood at 1 dpi (C) and 14 dpi (D). E, G, Quantitation of PMN absolute cell numbers per 5000 events demonstrated that the hCNS-SCns + anti-Ly6G treatment group had depleted numbers of blood PMNs at 1 dpi (E) and 14 dpi (G). F, H, In contrast, the quantitation of monocyte absolute cell number per 5000 events demonstrated no difference in the number of blood monocytes between hCNS-SCns + IgG2a and hCNS-SCns + anti-Ly6G groups at either 1 dpi (F) or 14 dpi (H). C–H, Quantitative flow cytometry results for groups that received injections with hCNS-SCns (open circles) or vehicle (filled squares) for both IgG2a (green) and anti-Ly6G (purple) in the same graphs for reference. No significant differences in the percentage or absolute number of PMNs or monocytes were detected between groups that received hCNS-SCns versus vehicle within either the IgG2a or anti-Ly6G treatment groups. N = 10–12′, ***p < 0.001, Student's t test. Values are given as the mean ± SEM.