Figure 2.

gk1234 Mutation fails to complement vt32 mutation, generates DA-dependent Swip. A, Like swip-13(vt32) animals, swip-13(gk1234) show significant Swip, and this behavior is also rescued by reserpine pretreatment (t₍₁₉₀₎ = 13.3, p > 0.0001, Bonferroni's post-tests). Data were analyzed using a one-way ANOVA with Bonferroni's post-tests comparing each genotype without reserpine to the same genotype with reserpine. Significance was set at p < 0.05. *p < 0.01, ****p < 0.0001. B, Heterozygous vt32/+ and gk1234/+ animals swim normally, but double-heterozygous gk1234/vt32 animals demonstrate significant paralysis. Data were analyzed using a one-way ANOVA with Bonferroni's post-tests comparing gk1234/vt32 double-heterozygotes to both vt32/+ (t₍₆₉₎ = 8.55, p < 0.0001, Bonferroni's post-tests) and gk1234/+ (t₍₆₉₎ = 9.76, p < 0.0001, Bonferroni's post-tests) single-heterozygotes. Significance was set at p < 0.05. ****p < 0.0001. C, Both dop-3(vs106) and cat-2(tm2261) mutations significantly suppress Swip in swip-13(gk1234) animals. Data were analyzed using a one-way ANOVA with Bonferroni's post-tests comparing swip-13(gk1234) to both swip-13(gk1234);dop-3(vs106) (t₍₁₉₇₎ = 18.77, p < 0.0001, Bonferroni's post-tests) and cat-2(tm2261);swip-13(gk1234) (t₍₁₉₇₎ = 24.76, p < 0.0001, Bonferroni's post-tests). Significance was set at p < 0.05. ****p < 0.0001. (D) Heat map analysis again shows a near complete rescue of Swip in swip-13(gk1234);dop-3(vs106) and cat-2(tm2261);swip-13(gk1234) animals.