Figure 4.

swip-13 Acts in DA neurons to regulate DA signaling. A, A fusion construct using 1 kb upstream of the swip-13 ATG start site fused to GFP drives GFP expression in swip-13-expressing cells. GFP (green) is observed in a number of neurons in the (A) head, (B) body, and (E) tail of the animal. Coexpression of Pdat-1::mCherry (red) to label DA neurons shows complete colocalization of swip-13-driven GFP and DA neurons (arrows). This is shown by yellow overlap in the CEP and ADE neurons in the head (C) and in the PDE neurons in the body (D). All images were acquired with a 63× objective and represent compressed z-stacks. F, A transgene driving genomic swip-13 by the dat-1 promoter (Pdat-1::swip-13) was made to restrict swip-13 expression to DA neurons. swip-13(gk1234) animals expressing this transgene showed a significant suppression of Swip compared with nontransgenic swip-13(gk1234) animals (t₍₇₃₎ = 8.34, p < 0.0001, unpaired t test). Bars represent the average of three transgenic lines with at least 100 animals per line. Significance was calculated using a two-tailed Student's t test with significance set at p < 0.05. ****p < 0.0001.