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. 2017 Sep 20;37(38):9305–9319. doi: 10.1523/JNEUROSCI.1363-17.2017

Figure 4.

Figure 4.

Simultaneous single-trial recordings from multiple neurons. Left, Resting light image. Right, hVOS traces. Selected cells were numbered to correspond with the traces and the ROIs for each cell were outlined. A, hVOS responses in five different parvalbumin interneurons in layer 2/3 of the somatosensory cortex. Stimulus site is just dorsal to (below) the field of view. B, hVOS responses in five interneurons of layer 5 in the somatosensory cortex of an hVOS::GAD2 mouse. Blue traces from two additional ROIs represent that the background fluorescence produces no fluorescence change (circle) or a small fluorescence change (square). The small fluorescence change likely arises from voltage changes in processes from that location. Stimulus site is dorsal to (above) the field of view. C, Newborn granule cells in the DG of an hVOS::Nestin mouse responding to outer molecular layer stimulation (site indicated by open circle). D, hVOS responses in five neurons in layer 2/3 of the entorhinal cortex of an hVOS::Calb2 mouse. A trace from a region without a labeled neuron (blue) shows no fluorescence change. Stimulus site is caudal to the field of view (left). E, hVOS responses in three neurons from the hilus in the DG of an hVOS::CalCrl mouse. A trace from a region without a labeled neuron (blue) shows no fluorescence change. Stimulus site is in the middle molecular layer, indicated by an open circle. The intense fluorescence in the inner molecular layer arises from mossy cell axons. F, hVOS responses in five neurons from layer 2/3 the somatosensory cortex of an hVOS::CalCrl mouse. Stimulation in layer 2/3 (left) evoked EPSPs. G, hVOS responses in 12 neurons in the DG of a hippocampal slice from an hVOS::Fos mouse expressing hVOS probe in granule cells. Stimulation was applied to the outer molecular layer. The mouse had been placed in a novel environment as described in Materials and Methods. These neurons were presumably active in that environment. All traces are single trials; stimulus: 200 μA, 180 μs.