Table 1.
Primers and probes used in fluorescent in-situ Hybridization (FISH) and Fluorescence in-situ Hybridization with Tyramide Signal Amplification (TSA-FISH).
| Oligonucleotide | Target gene | Specificity | Sequence | Reference |
|---|---|---|---|---|
| Probes for FISH: | ||||
| EUB338 (antisense) | 16S rRNA |
Eubacteria | 5′GCTGCCTCCCGTAGGAGT3′ | Amann et al., 1990 |
| EUB II (antisense) | 16S rRNA |
Planctomycetales | 5′GCAGCCACCCGTAGGTGT3′ | Amann et al., 1990 |
| Rick-B1 (antisense) | 16S rRNA |
Rickettsia | 5′CCATCATCCCCTACTACA3′ | Perotti et al., 2006 |
| NONRickettsia (sense) | 16S rRNA |
Rickettsia | 5′TGTAGTAGGGGATGATGG3′ | Kuchler et al., 2009 |
| Primers used for generating DIG-labeled ssDNA probes in TSA-FISH: | ||||
| Antisense primer | ompA | Rickettsia | 5′GTTCCGTTAATGGCAGCATCT3 | Roux et al., 1996 |
| Sense primer | ompA | Rickettsia | 5′ATGGCGAATATTTCTCCAAAA3′ | Roux et al., 1996 |