Fig. 1.
Product formation in whole cells over 48 h of biotransformation. Low cell density was between 3.5 and 5 gDCW/L. High cell density was >25 gDCW/L. High cell density cultures produced octyl acetate by-product alongside 1-octanol (an artefact of the expression system). The product concentrations shown here are the combined concentrations of 1-octanol and octyl acetate. Two vials were sacrificed to obtain each sample point. The organic phase was extracted into ethyl acetate and analysed via gas chromatography