Figure 2.

The two variants did not block the transport of neuroligin from ER to cell membrane. HEK293 cells expressing GFP‐tagged wild‐type neuroligin (NL3‐WT and NL4‐WT), mutant neuroligin (NL3‐G426S and NL4‐Q162K), and positive control (NL3‐R451C and NL4‐R87W) were immunostained with antibodies against the GFP tag (first column, green) and against the Golgi‐marker GM130 (second column, red). The nucleus was labeled by DAPI (4′,6‐diamidino‐2‐phenylindole) (third column, blue). Both the wild‐type and mutant neuroligin are transported efficiently to the cell surface, whereas the positive control accumulates in the Golgi body, as confirmed by the overlap with the Golgi body‐resident protein calnexin. The scale bar is 5 mm