Fig. 1.
2-APB prevented IL-4-induced Ca2+ influx in BECs. Intracellular Ca2+ ([Ca2+]i) levels were recorded using Fura-2/AM. The fluorescence of Fura-2 was detected in cells with a stimulation of IL-4 (20 ng/ml) in the presence or absence of 50 μM 2-APB. Ca2+ signals were recorded for the calculation of fluorescence intensity ratio of F340/F380. (a-d) Representative traces of calcium responses. (e and f) Changes in duration and amplitude of [Ca2+]i. Results are expressed as mean ± SD; n = 10. *P < 0.05 versus BSA, # P < 0.05 versus IL-4 treated group