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. 2017 Sep 20;18:174. doi: 10.1186/s12931-017-0657-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Blocking lipid rafts aggregation prevented Ca²⁺ influx induced by IL-4. Intracellular Ca²⁺ levels were recorded using Fura-2/AM. BECs were pretreated with or without 10 mM M-βCD. Then, the fluorescence of Fura-2 was detected in cells with a stimulation of 20 ng/ml IL-4. Ca²⁺ signals were recorded for calculating the fluorescence intensity ratio of F340/F380. (a-d) Representative trace of calcium responses. (e and f) Changes in Ca²⁺ influx duration and amplitude. Results are expressed as mean ± SD; n = 12. *P < 0.05 versus BSA, ^# P < 0.05 versus IL-4 treated group