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. 2017 Sep 20;17:158. doi: 10.1186/s12870-017-1101-8

Fig. 1.

Fig. 1

Diagrams of PCR amplification for Pina and Pinb. The gray boxes show coding regions for Pina and Pinb, respectively. The first step PCR reaction of Pina and Pinb (Pina-OutF/R, Pinb-OutF/R) used for amplifying Pina (1933 bp) and Pinb (1916 bp) genomic sequence. The second step PCR reaction of Pina and Pinb (Pina-InF/R, Pinb-InF/R) used for amplifying Pina (1239 bp) and Pinb (1421 bp) genomic sequence