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a Specificity evaluation of the RT-LAMP assay determined by digestion with restriction enzyme SmlI (a); lane 1, positive control, lane 2, LAMP products from lane 1 after digestion with SmlI; M, DL500 DNA marker; specificity of the RT-LAMP assay determined by electrophoresis analysis (b) and SYBR Green I staining (c); lane 1, PDCoV; lane 2–9, PEDV, TGEV, PKoV, PAstV, PRRSV, CSFV, PCV2, and negative control
