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. 2017 May 3;(123):55418. doi: 10.3791/55418
0.05 M PBS 2 L DI water:
Store in glass beaker wrapped with tin foil. Bring pH to 7.4 with KOH if necessary. NaH2PO4·H2O: 2.8 g Na2HPO4: 11.34 g Sodium Chloride: 11.68 g
20 mM Ascorbic Acid 50 mL DI water:
Make fresh daily. Ascorbic acid: 0.18 g
20 mM L-Glutamate 50 mL DI water:
Make fresh weekly. L-glutamate monosodium salt hydrate: 0.15 g
2 mM Dopamine Dopamine hydrochloride: 0.038 g
Make fresh monthly. 0.1 M Perchloric acid: 10 mL Bring volume to 100 mL with DI water
8.8 mM Hydrogen Peroxide 50 mL DI water:
Make fresh weekly. Hydrogen peroxide: 500 μL
70 mM potassium chloride 100 mL DI water:
Make fresh day of experiment. Potassium chloride: 0.08 g Sodium chloride: 0.07 g Calcium Chloride: 0.004 g
200 μM Glutamate 20 mM glutamate: 100 μL
Make fresh day of experiment. Sterile saline: 10 mL