0.05 M PBS
|
2 L DI water: |
Store in glass beaker wrapped with tin foil. Bring pH to 7.4 with KOH if necessary. |
NaH2PO4·H2O: 2.8 g Na2HPO4: 11.34 g Sodium Chloride: 11.68 g |
20 mM Ascorbic Acid
|
50 mL DI water: |
Make fresh daily. |
Ascorbic acid: 0.18 g |
20 mM L-Glutamate
|
50 mL DI water: |
Make fresh weekly. |
L-glutamate monosodium salt hydrate: 0.15 g |
2 mM Dopamine
|
Dopamine hydrochloride: 0.038 g |
Make fresh monthly. |
0.1 M Perchloric acid: 10 mL Bring volume to 100 mL with DI water |
8.8 mM Hydrogen Peroxide
|
50 mL DI water: |
Make fresh weekly. |
Hydrogen peroxide: 500 μL |
70 mM potassium chloride
|
100 mL DI water: |
Make fresh day of experiment. |
Potassium chloride: 0.08 g Sodium chloride: 0.07 g Calcium Chloride: 0.004 g |
200 μM Glutamate
|
20 mM glutamate: 100 μL |
Make fresh day of experiment. |
Sterile saline: 10 mL |