Excitation laser | Fluorochrome | Specificity | Clone | |
488 nm | BB515 | CD8 | 53-6.7 | |
488 nm | FITC | Ly49D | 4E5 | |
488 nm | PE | F4/80 | 6F12 | |
488 nm | PE-CF594 | NK1.1 | PK136 | |
488 nm | PE-Cy5 | CD44 | IM7 | |
488 nm | PE-Cy7 | CD11b | M1/70 | |
488 nm | PerCP-Cy5.5 | IgM | R6-60.2 | |
488 nm | PerCP | Gr-1 | RB6-8CS | |
488 nm | AF532 | CD3 | 17A2 | |
488 nm | Propidium Iodide | Viability | ||
405 nm | V450 | IgD | 11-26c.2a | |
405 nm | BV421 | CD11c | HL3 | |
405 nm | BV510 | CD19 | 1D3 | |
405 nm | BV570 | TCRb | H57-597 | |
405 nm | BV605 | CD4 | RM4-5 | |
405 nm | BV650 | CD45R/B220 | RA3-6B2 | |
405 nm | BV711 | I-A/I-E | M5/114 | |
405 nm | BV786 | CD25 | PC61 | |
405 nm | eVolve 655 | CD45 | 30-F11 | |
N/A | purified | CD16/CD32 | 2.4G2 | Fc-receptor block |
List of Antibodies used in cardiac cell suspension | ||||
638 nm | Alexa Fluor 647 | CD31 | MEC13.3 | |
488 nm | PE | CD45 | 30-F11 | |
488 nm | PE-Cy5 | Sca-1 | D7 | |
488 nm | PE | Ter119 | TER-119 |