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. 2017 May 5;(123):55578. doi: 10.3791/55578
Excitation laser Fluorochrome Specificity Clone
488 nm BB515 CD8 53-6.7
488 nm FITC Ly49D 4E5
488 nm PE F4/80 6F12
488 nm PE-CF594 NK1.1 PK136
488 nm PE-Cy5 CD44 IM7
488 nm PE-Cy7 CD11b M1/70
488 nm PerCP-Cy5.5 IgM R6-60.2
488 nm PerCP Gr-1 RB6-8CS
488 nm AF532 CD3 17A2
488 nm Propidium Iodide Viability
405 nm V450 IgD 11-26c.2a
405 nm BV421 CD11c HL3
405 nm BV510 CD19 1D3
405 nm BV570 TCRb H57-597
405 nm BV605 CD4 RM4-5
405 nm BV650 CD45R/B220 RA3-6B2
405 nm BV711 I-A/I-E M5/114
405 nm BV786 CD25 PC61
405 nm eVolve 655 CD45 30-F11
N/A purified CD16/CD32 2.4G2 Fc-receptor block
List of Antibodies used in cardiac cell suspension
638 nm Alexa Fluor 647 CD31 MEC13.3
488 nm PE CD45 30-F11
488 nm PE-Cy5 Sca-1 D7
488 nm PE Ter119 TER-119