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. Author manuscript; available in PMC: 2017 Sep 21.
Published in final edited form as: Am J Transplant. 2015 Feb 3;15(7):1933–1947. doi: 10.1111/ajt.13185

Figure 1. Study design.

Figure 1

The workflow of this study was performed in three steps: (1) global expression profiling of PBMC miRNA pools extracted from three pools of LT recipients (stable, DSA+BOS– and DSA+BOS+) using TaqMan Low-Density Human miRNA Array, in combination with Megaplex RT and Megaplex pre-amplification techniques; (2) analysis and preliminary evaluation of candidate miRNAs by heatmap, potential target prediction and pathway enrichment; and (3) independent validation of differentially expressed miRNAs.