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. 2017 May 22;(123):55497. doi: 10.3791/55497
intra. extra.1 extra.2
high chloride high chloride low chloride
c  [mM] c  [mM] c  [mM]
Na-ASP 0 0 140
NaCl 110 140 0
KCl 1 1 1
CsCl 1 1 1
CaCl2 2 2 2
MgCl2 2 2 2
HEPES 10 10 10
EGTA 10 0 0
pH 7.2 7.2 7.2
osmolarity  290 mOsm 320 mOsm 320 mOsm