Component	Amount per reaction (µL)	Final
First-strand cDNA	6
Single strand DNA ligase buffer (10x)	1	1x
Betaine (5 M)	2	1 M
MnCl2 (50 mM)	0.5	2.5 mM
Single strand DNA ligase (100U/µL)	0.5	5 U/µL
Total	10