| Step | Temperature | Time | Cycles |
| Initial Denaturation | 98 °C | 30 s | 1 |
| Denaturation | 98 °C | 10 s | |
| Annealing | 65 °C | 30 s | 25 |
| Extension | 72 °C | 30 s | |
| Final Extension | 72 °C | 5 min | 1 |
| Hold | 4 °C | ∞ |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.