| Reagents | Volume (µL) | Final |
| PNK reaction | 80 | |
| Nuclease free water | 3 | |
| 10x T4 PNK buffer | 2 | 1x |
| 10 mM ATP | 10 | 1 mM |
| T4 PNK (10 U/µL) | 5 | 0.5 U/µL |
| Total | 100 |
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