| Component | Amount per reaction (µL) | Final |
| RNA and linker | 5 | |
| T4 Rnl2 buffer (10x) | 1 | 1x |
| PEG 8000 (50%, wt/vol) | 3 | 15% (wt/vol) |
| Rnase inhibitor (20 U/µL) | 0.5 | 1 U/µL |
| T4 Rnl2 (tr) (200U/µL) | 0.5 | 10 U/µL |
| Total | 10 |
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