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. 2017 May 29;(123):55537. doi: 10.3791/55537
Concept Mean (n = 4) S.D. Comments
Initial 18F activity (mCi) 148.0 44.9 Start of synthesis
Precursor amount (μg) 50 Use 50 μL of 1.0 mg/mL stock
Activity left in QMA (mCi) 3.0 1.7 Measured at the end of labeling step
Radiolabeling yield 29.7% 6.3% Act_collection_vial ÷ (Act_collection_vial + Act_AluN)
Radiochemical purity (HPLC-1) > 98% From HPLC-1 QC
Spec. act. intermediate (mCi/μmol) 122.9 29.7 From HPLC-1 using calibration curve
Hydrogenation recovery (d.c.) 74% 9.0% Corrected for decay
HPLC radiochemical purity (HPLC-2) 90.7% 2.9% Calculated from HPLC-2
Drying efficiency > 98% Corrected for decay
Filtering recovery 93.5% 1.7% Corrected for decay
Dose volume (mL) 3.3 Collect fractions with highest radioactivity
Spec. act. final product (mCi/µmol) 75.5 30.0 From HPLC-3 using calibration curve
Synthesis efficiency 8.5% 3.6% Non-decay corrected
Synthesis time (min) 104 11.2