Time (min)	% Buffer B to Buffer A
0 to 5 min	Linear gradient from 0 to10%	Buffer A: 0.1% formic acid in UPLC grade water
5 to 80 min	Linear gradient from 10% to 40%	Buffer B: 0.1% formic acid in 60% UPLC grade acetonitrile
80 to 85 min	Linear gradient from 40% to 60%	Flow rate 300 nL/min
85 to 86 min	Linear gradient from 60%  to 95%	Injection volume 5 µL
86 to 91 min	Wash column for 5 min with 95%
91 to 92 min	Linear gradient from 95%  to 0%
93 to 110 min	Equilibrate the column for 17 min at 0%