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. 2017 Sep 21;12(9):e0184584. doi: 10.1371/journal.pone.0184584

Table 3. qRT-PCR validation of transcriptome sequencing.

Functional classification ID Function RNA-seq qRT-PCR
Transcription M1 Sox-14 166.664 170.386
M2 Armadillo repeat-containing protein 4 11.295 53.269
M3 Armadillo repeat-containing protein 3 6.678 14.705
M4 Sox-8 533.812 2987.417
M5 forkhead box J1 protein, partial 79.224 3.825
F1 Foxl2 129.687 146.537
F2 Forkhead box protein N2 115.624 133.897
F3 Forkhead box E protein, partial 47.888 117.538
F4 Spermatogenesis- and oogenesis
-specific protein 2
23.519
A1 (M) Sox2 5.920 1.412
A2 (F) β-catenin 2.203 4.045
A3 (F) Dax1 1.344 1.766
A4 (M) Sox 9 1.593 2.174
A5 (M) DMRTA2 2.468 29.468
Signal transduction mechanisms
M6 Testis-specific serine/threonine-protein kinase 1 2264.369 1482.427
M7 Troponin C, skeletal muscle 411.277 5803.698
M8 Testis-specific serine/threonine-protein kinase 1 1939.053 1056.228
M9 Sperm motility kinase X 334.901 3955.033
M10 Testis-specific serine/threonine-protein kinase 5 280.606
M11 Testis-specific serine/threonine-protein kinase 4 1832.153 4594.291
Carbohydrate, Lipid, Amino acid transport and metabolism M12 Glycogen phosphorylase, muscle form 1523.181 40322.961
M13 Tax1-binding protein 1-like protein B 163.139 73.838
F5 Vitellogenin-6 2090.712
F6 Chymotrypsin-like elastase family member 2A 17.829 2.962
F7 Chymotrypsin-like serine proteinase 17.423 7.342
F8 Chymotrypsin-like elastase family member 2A 14.892 4.283
F9 Chymotrypsin-like serine proteinase 16.022 5.073
Egg coated protein F10 vitelline envelope zona pellucida domain 4 129.305
F11 vitelline envelope zona pellucida domain 10 12.964 7.139
F12 vitelline envelope zona pellucida domain 10 597.534 177.465
F13 vitelline envelope zona pellucida domain 10 12.382 3.310
Immune-related protein M14 Sperm-associated antigen 6 20.916 41.411
F14 placenta-specific gene 8 protein-like 20.263 31.953
F15 Placental protein 11 7.582 10.440
Cell cycle control M15 F-box only protein 39 350.894 6.619
F16 G2/mitotic-specific cyclin-B 193.215 159.333
Chromatin structure and dynamics M16 Sperm-specific protein PHI-2B/PHI-3 716.255 2678.482

‘–’ represents male or female-specific genes, M represents males, F represents females and fold change indicates the differential change in expression between the two genders.