Table 3. qRT-PCR validation of transcriptome sequencing.
Functional classification | ID | Function | RNA-seq | qRT-PCR |
---|---|---|---|---|
Transcription | M1 | Sox-14 | 166.664 | 170.386 |
M2 | Armadillo repeat-containing protein 4 | 11.295 | 53.269 | |
M3 | Armadillo repeat-containing protein 3 | 6.678 | 14.705 | |
M4 | Sox-8 | 533.812 | 2987.417 | |
M5 | forkhead box J1 protein, partial | 79.224 | 3.825 | |
F1 | Foxl2 | 129.687 | 146.537 | |
F2 | Forkhead box protein N2 | 115.624 | 133.897 | |
F3 | Forkhead box E protein, partial | 47.888 | 117.538 | |
F4 | Spermatogenesis- and oogenesis -specific protein 2 |
23.519 | — | |
A1 (M) | Sox2 | 5.920 | 1.412 | |
A2 (F) | β-catenin | 2.203 | 4.045 | |
A3 (F) | Dax1 | 1.344 | 1.766 | |
A4 (M) | Sox 9 | 1.593 | 2.174 | |
A5 (M) | DMRTA2 | 2.468 | 29.468 | |
Signal transduction mechanisms |
M6 | Testis-specific serine/threonine-protein kinase 1 | 2264.369 | 1482.427 |
M7 | Troponin C, skeletal muscle | 411.277 | 5803.698 | |
M8 | Testis-specific serine/threonine-protein kinase 1 | 1939.053 | 1056.228 | |
M9 | Sperm motility kinase X | 334.901 | 3955.033 | |
M10 | Testis-specific serine/threonine-protein kinase 5 | 280.606 | — | |
M11 | Testis-specific serine/threonine-protein kinase 4 | 1832.153 | 4594.291 | |
Carbohydrate, Lipid, Amino acid transport and metabolism | M12 | Glycogen phosphorylase, muscle form | 1523.181 | 40322.961 |
M13 | Tax1-binding protein 1-like protein B | 163.139 | 73.838 | |
F5 | Vitellogenin-6 | 2090.712 | — | |
F6 | Chymotrypsin-like elastase family member 2A | 17.829 | 2.962 | |
F7 | Chymotrypsin-like serine proteinase | 17.423 | 7.342 | |
F8 | Chymotrypsin-like elastase family member 2A | 14.892 | 4.283 | |
F9 | Chymotrypsin-like serine proteinase | 16.022 | 5.073 | |
Egg coated protein | F10 | vitelline envelope zona pellucida domain 4 | 129.305 | — |
F11 | vitelline envelope zona pellucida domain 10 | 12.964 | 7.139 | |
F12 | vitelline envelope zona pellucida domain 10 | 597.534 | 177.465 | |
F13 | vitelline envelope zona pellucida domain 10 | 12.382 | 3.310 | |
Immune-related protein | M14 | Sperm-associated antigen 6 | 20.916 | 41.411 |
F14 | placenta-specific gene 8 protein-like | 20.263 | 31.953 | |
F15 | Placental protein 11 | 7.582 | 10.440 | |
Cell cycle control | M15 | F-box only protein 39 | 350.894 | 6.619 |
F16 | G2/mitotic-specific cyclin-B | 193.215 | 159.333 | |
Chromatin structure and dynamics | M16 | Sperm-specific protein PHI-2B/PHI-3 | 716.255 | 2678.482 |
‘–’ represents male or female-specific genes, M represents males, F represents females and fold change indicates the differential change in expression between the two genders.