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. 2017 Sep 21;12(9):e0184473. doi: 10.1371/journal.pone.0184473

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© 2017 Qian et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Skeletal staining of the rib cages of conditional Pdgfra knockout (CKO, Pdgfra^Cre/ERT;Pdgfra^fl/fl) (A-D) and control (Ctrl, Pdgfra^fl/fl) (E) embryos were shown for comparison. The ribs were numbered and arrows indicated the respective location of the manubrium. CKO embryos developed spina bifida (arrowhead), and the vertebral arches of the CKO vertebrae failed to form the convex structure as observed in the control littermate (F). Abbreviations: m, manubrium; st, sternum; xp, xiphoid process; vb, vertebral body; ar, vertebral arch.