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. 2017 Sep 21;12(9):e0184473. doi: 10.1371/journal.pone.0184473

Fig 7. Conditional knockout of Pdgfra in Pdgfra-expressing tissues at E10.5 resulted in milder developmental anomalies.

Fig 7

PdgfraCre/ERT;Pdgfrafl/+ mice were crossed with Pdgfrafl/fl, tamoxifen was given to pregnant females at E10.5 for two consecutive days. Conditional knockout (CKO, PdgfraCre/ERT;Pdgfrafl/fl) and control (Ctrl, Pdgfrafl/fl) embryos were collected at E18.5 for analysis. Morphological examinations of CKO embryos (A, B) and control littermate (C). Haematoxylin and eosin staining of sagittal sections of the head of CKO embryos (D, E) and control littermate (F). Von Kossa staining (black) of the sagittal sections of the head of CKO embryos (H, I) and control littermate (J). Highlighted regions were magnified and shown on the right, and arrowheads indicated the positive staining of bone. Arrows indicated omphalocele. Abbreviations: tg, tongue; md, mandible; mx, maxilla; pa, palatal shelf; bp, basisphenoid bone; bo, basioccipital bone; nb, nasal bone; ns, nasal septum; hy, hyoid bone; so, supraoccipital bone; ep, epidermis; de, dermis; br, brain; bo, bone.