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A and B. SDS-PAGE and zinc-imidazole stained midi gel. (A) SDS-PAGE of two elution fractions of the cobalt-affinity chromatography in lane 1 and 2. In lane M the marker is shown. HPRG migrates on SDS-Gel at approximately 90 kDa. Minor proteolytic fragments with lower molecular weight are present. In lane 2 the lower band may also correspond to serum albumin (B) Zinc-imidazole stained gel after preparative gel electrophoresis. The thick band corresponds to the native HPRG and is cut out.
