Table 1. Acetonitrile-Water gradient applied to the reversed phase HPLC.

time / min	solvent A / %	solvent B / %
0–6	90	10
6–7	90–70	10–30
7–20	70–57	30–43
20–20.3	57–40	43–60
20.3–25	40	60
25–27	40–10	60–90
27–30	10	90
30–32	10–90	90–10
32–40	90	10

Acetonitrile-Water gradient applied to the reversed phase HPLC to purify HPRG from remaining buffer salts and SDS after preparative gel electrophoresis (solvent A: water + 0,05% TFA; solvent B: acetonitrile + 0,05% TFA). The sudden jump from 43% to 60% acetonitrile at 20.3 min focuses the protein to few elution fractions.