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. 2017 Sep 21;12(9):e0185088. doi: 10.1371/journal.pone.0185088

Fig 1. EMT and MDR appears in sorafenib-resistant HCC cells.

Fig 1

A, The sorafenib-resistant HepG2-SR and Huh7-SR cells and parental HepG2 and Huh7 cells were incubated with increasing concentrations of sorafenib for 48 hours. Cell viability (%) was assessed and compared with the corresponding untreated cells. B, Morphological changes were evaluated in and sorafenib-resistant cells under ×400 magnification. C, Migration of HCC cells was measured by with a Transwell assay. D, Invasion of HCC cells was also measured with a Transwell assay. E, EMT- and MDR-related proteins, including E-cadherin, Vimentin, MMP-9, Snail and P-gp, were assessed by western blotting in parental and resistant cells. β-actin was used as a loading control in the western blot analysis. Data represent three independent experiments. ** indicates P<0.001.