A, The sorafenib-resistant HepG2-SR and Huh7-SR cells were transfected with Snail siRNA and MDR siRNA, and cells were then incubated with increasing concentrations of sorafenib for 48 hours. Cell viability (%) was assessed and compared with the corresponding untransfected cells. B, Knockdown of snail abolished the metastatic potential of HepG2-SR cells. C, Knockdown of snail abolished the invasive ability of HepG2-SR cells. D, HepG2-SR cells were transfected with control or Snail and/or MDR1 siRNA for 24 hours, and the protein levels of EMT and MDR markers were detected by western blot. β-actin served as the loading control in the western blot analysis. Data represent three independent experiments. N.S., not significant. * indicates P<0.05, and ** indicates P<0.001.