Table 2. Description of candidate reference genes and primer sequences for qRT-PCR.
| MDP# Gene description |
Primers F/R [5’-3’] | Amplicon size (bp) | Tm (°C) | PCR efficiency (%) | Regression coefficiency (R2) |
|---|---|---|---|---|---|
| MDP0000095375 Casein kinase II subunit beta-4 isoformX2 |
F-5’ ACATCGAGAGCTTTGGCTAAC 3’ R-5’ CTCCATCAGAACCACTGACATC 3’ |
187 | 82.0 | 98.75 | 0.9993 |
| MDP0000121104 WD repeat-containing protein DWA2 |
F-5’ GCATTTGATTCGGCTCTCTTC 3’ R-5’ GCTCAGGGATCTGCCATATT 3’ |
169 | 84.5 | 100.83 | 0.9982 |
| MDP0000147424 Protein GRIP |
F-5’ CTCAGGCTGATCGCAATGATA 3’ R-5’ CCAGCTCCTCAATTTCCTCTT 3’ |
168 | 84.5 | 105.35 | 0.9970 |
| MDP0000173025 Membrane related |
F-5’ ATGGAGAGATGGAATGGCAAAG 3’ R-5’ GTGAGCATCGGATCCCATTTAG 3’ |
193 | 85.0 | 92.92 | 0.9992 |
| MDP0000200579 Ubiquitin-conjugating enzyme E223 |
F-5’ CCTAATCGTGTGGAAGGTACTG 3’ R-5’ ATGAGGGAAGAAGACACCAATC 3’ |
192 | 82.0 | 100.39 | 0.9998 |
| MDP0000211280 26S proteasome non-ATPase regulatory subunit 6 |
F-5’ TCTGTTACCAAGATCGCTGATG 3’ R-5’ ATGGAAGACCAAGTCCATCTTT 3’ |
179 | 83.5 | 97.52 | 0.9998 |
| MDP0000233640 Ribosome biogenesis protein BMS1 |
F-5’ CAAGGCCGAGAAGAAGAAGAA 3’ R-5’ TCCATGAACCAGAACGACATAC 3’ |
198 | 88.0 | 101.61 | 0.9968 |
| MDP0000245145 Poly(U)-specific endoribonuclease-B |
F-5’ GGGCCAGTGGAATGGATATAAG 3’ R-5’ CCCTCCACCACAGTCAATTT 3’ |
189 | 83.0 | 101.78 | 0.9996 |
| MDP0000314861 PRA1family protein A1-like |
F-5’ TCTCATCGCAGCTGTTCTTAC 3’ R-5’ GAGGCCGTCCACATATGAAT 3’ |
196 | 82.5 | 97.80 | 0.9988 |
| MDP0000315565 Trafficking protein particle complex subunit 12 |
F-5’ GATCGGCTGTACTGCTTGTTAG 3’ R-5’ GGGTCTGTGTAATCACGGTTAAG 3’ |
193 | 81.5 | 108.20 | 0.9986 |
| MDP0000326399 Glycerol-3-phosphate acyltransferase 3 |
F-5’ CGTGGAACTGGAATGTGTATCT 3’ R-5’ ACTCTACCAAGAGCCTCTCTAA 3’ |
195 | 85.5 | 94.01 | 0.9963 |
| MDP0000336547 SGF 29 tudor-like domain-containing protein |
F-5’ AGCTACTCCTGAAGTGGTAGAG 3’ R-5’ GCTCATTACCTTCCATCCTTCTT 3’ |
188 | 85.0 | 106.96 | 0.9994 |
| MDP0000752428 Actin like |
F-5’ AGTCCAAGCGTGGTATCTTAAC 3’ R-5’ CTGGGTCATCTTCTCACGATTT 3’ |
188 | 87.0 | 98.97 | 0.9982 |
| MDP0000831483 Transcription factor GTE8-like isoformX1 |
F-5’ GGACTCTGGTGAGGATGAGATC 3’ R-5’ CCGGGTCATTCCCTTTACAT 3’ |
199 | 80.5 | 104.17 | 0.9977 |
| MDP0000913709 Ubiquitincarboxyl-terminal hydrolase isozymeL5-like |
F-5’ GCAGAAGGTAGCTGACAAAGA 3’ R-5’ TCGATGCGTTCCTGGATTAC 3’ |
182 | 85.0 | 102.25 | 0.9997 |
MDP: predicted gene model from the apple (Malus x domestica) genome sequences v3.0.a1 (https://www.rosaceae.org/analysis/162). Gene description is based the annotation by BlastP against NR (nonredundant protein sequences) database.