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. 2017 Sep 21;12(9):e0185250. doi: 10.1371/journal.pone.0185250

Fig 5. Attenuated secretion of glomerular Esm-1 with diabetes/high glucose in DN-susceptible compared to DN-resistant mice.

Fig 5

(A) Glomeruli were isolated from mice treated with vehicle or streptozotocin for 4 weeks and cultured for 24 hours. Mouse Esm-1 was measured by ELISA from conditioned media. Each circle represents data from one mouse. Open and closed circles indicate data from vehicle-injected and streptozotocin-injected (i.e. control and diabetic) mice, respectively. Ctrl, control; DM, diabetic. (B-C) Glomeruli were isolated from mice treated with vehicle only and cultured in low (100 mg/dL) or high glucose (450 mg/dL) for 24 hours. Mean Esm-1 mRNA (B) and secreted protein (C) were compared between low (open circles) and high glucose (closed circles) pairwise from individual mouse glomeruli. Each circle represents the group mean. Red and black circles/asterisks indicate data from DN-susceptible and DN-resistant mice, respectively. *, p-value < 0.05 in the same mouse strain between control and diabetic or between low and high glucose media groups; #, p-value < 0.05 in the same treatment between the two mouse strains. N = 6–7 mice per group; n = 3 replicate wells per sample in qPCR, and n = 2 replicate wells per sample in ELISA.