| Primer | Use | Sequence |
| OSC-F | OSCAR forward sequencing primer, sequences sense strand of 5’ flank | 5’-CTAGAGGCGCGCCGATATCCT |
| OSC-R | OSCAR reverse sequencing primer, sequences anti-sense strand of 5’ flank | 5’-CGCCAATATATCCTGTCAAACACT |
| HygR (210) | Reverse sequencing primer, sequences anti-sense strand of 5’ flank | 5’-GCCGATGCAAAGTGCCGATAAACA |
| HygF (850) | Forward sequencing primer, sequences sense strand of 3’ flank | 5’-AGAGCTTGGTTGACGGCAATTTCG |
| New Hyg Marker Forward | To amplify the hygromycin resistance gene as a control (together with New Hyg Marker Reverse primer below) | 5'-GACAGGAACGAGGACATTATTA |
| New Hyg Marker Reverse | To amplify the hygromycin resistance gene as a control (together with New Hyg Marker Forward primer above) | 5'-GCTCTGATAGAGTTGGTCAAG |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.