Fig 8. CD11c-specific HIF-1α-knockout mice produce more monocyte’s progenitors and display enhanced output of inflammatory monocytes in the bone marrow.

Hif^flox/flox-Cd11c-Cre⁺ and Cre^- mice were infected with L. donovani and sacrificed at various time point of infection. (A) Gating strategy to identify GMPs in the bone marrow of L.donovani infected mice. (B) Frequency (left graph) and absolute numbers (right graph) of GMPs in the bone marrow of mice over the course of infection. (C-E) Frequency (upper graph) and absolute numbers (lower graph) of granulocytes (C), Ly6C^hi monocytes(D), Ly6C^lo/int monocytes (E) in the bone marrow of infected mice. (F-G) Percentage of CCR2⁺ (F) and CXCR4⁺ (G) Ly6C^hi monocytes in the bone marrow. (H) Percentage of Sca-1⁺ Ly6C^hi monocytes in the bone marrow. (I) Percentage of MHCII⁺ Ly6C^hi monocytes in the bone marrow. All data represent mean ± SEM of one of 4 independent experiments, n = 4. * denotes p<0.05.