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Plate readout of ex-vivo IFN-γ ELISpot responses to OT-WCA from frozen PBMC of rhesus macaques 28 days after infection with 106 O. tsutsugamushi (left) and non-infected control macaques (right) at 3 concentrations of 1.41 μg (dilution of 1:50), 0.71 μg (1:100) and 0.14μg (1:500) per well. PHA and ‘media’ are positive and negative control, respectively.
