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. 2017 Sep 11;11(9):e0005846. doi: 10.1371/journal.pntd.0005846

Table 1. Optimization of the ex vivo IFN-γ ELISpot assay.

Adjusted IFN-γ Spot Count (SFC/106 PBMC)
OT infected Controls
Well #1 Well #2 Mean 95%CI Well #1 Well #2 Mean 95%CI
PHA 155 195 175 (0–429) 440 405 422.5 (200–645)
Media 0 0 0 (0–0) 10 0 5 (0–69)
OT 1.41 μg 805 990 897.5 (0–207) 0 5 2.5 (0–34)
OT 0.705 μg 740 740 740 (740–740) 10 0 5 (0–69)
OT 0.141 μg 655 565 610 (38–1182) 15 5 10 (0–34)

Note: Spot counts are expressed as spot forming cells per million peripheral blood mononuclear cells (SFC/106 PBMC).