Table 2. Adjusted spot counts from ex-vivo IFN-γ ELISpot at each time point.
| Adjusted spot count (SFC/106 PBMC) | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Days after infection | Control macaques (n = 5) | O. tsutsugamushi infected macaques (n = 10) | |||||||||||||||
| 1 | 2 | 3 | 4 | 5 | Mean | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | Mean | |
| Day 0 | 7.5 | 12.5 | 7.5 | 12.5 | 32.5 | 14.5 | 0 | 15 | 2.5 | 12.5 | 10 | 12.5 | 7.5 | 17.5 | 15 | 25 | 11.75 |
| Day 7 | 2.5 | 22.5 | 10 | 0 | 12.5 | 9.5 | 262.5 | 232.5 | 192.5 | 252.5 | 262.5 | 212.5 | 240 | 302.5 | 267.5 | 125 | 235 |
| Day 14 | 7.5 | 30 | 0 | 0 | 55 | 18.5 | 1250 | 1392.5 | 1500 | 1500 | 997.5 | 1342.5 | 1245 | 1497.5 | 882.5 | 1005 | 1261.25 |
| Day 21 | 0 | 27.5 | 0 | 0 | 22.5 | 10 | 1497.5 | 1500 | 1500 | 1495 | 1500 | 1500 | 1492.5 | 1497.5 | 1497.5 | 1500 | 1498 |
| Day 28 | 22.5 | 25 | 7.5 | 15 | 0 | 14 | 1497.5 | 1500 | 1497.5 | 1577.5 | 1080 | 1480 | 1480 | 1310 | 1247.5 | 1495 | 1416.5 |
Values represent the mean SFC/106 PBMC count from cellular immune responses to OT-WCA after subtraction of the background response (complete media) after ID administration of live O. tsutsugamushi (n = 10) versus mock (n = 5) in rhesus macaques.