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. 2017 Jun 17;(124):55907. doi: 10.3791/55907
Name of the buffer Reagent Final concentration
Dilution buffer Sodium dodecyl sulfate (SDS) 0.01% (w/v)
Triton X-100 1.1% (v/v)
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 1.2 mM
Tris-HCl pH 8.1 16.7 mM
Sodium chloride (NaCl) 167 mM
DMA solution Dimethyl adipimidate (DMA) 10 mM
Phosphate-buffered saline (PBS) 1x
Elution buffer Sodium dodecyl sulfate (SDS) 1% (w/v)
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 10 mM
Tris-HCl pH 8.0 50 mM
High salt buffer Sodium dodecyl sulfate (SDS) 0.1% (w/v)
Triton X-100 1% (v/v)
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 2 mM
Tris-HCl pH 8.1 20 mM
Sodium chloride (NaCl) 500 mM
IMDM medium Iscove's modified Dulbecco's medium (IMDM) 1x
Fetal bovine serum (FBS) 2% (v/v)
Penicillin/Streptomycin 1x
Peptone primatone 0.3 mg/mL
Insulin solution human  4.8 mg/mL
Minimum essential medium non-essential amino acids (MEM NEAA) 1x
LiCl salt buffer Lithium chloride (LiCl) 0.25 M
IGEPAL-CA630 1% (v/v)
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 1 mM
Tris-HCl pH 8.1 10 mM
Low salt buffer Sodium dodecyl sulfate (SDS) 0.1% (w/v)
Triton X-100 1% (v/v)
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 2 mM
Tris-HCl pH 8.1 20 mM
Sodium chloride (NaCl) 150 mM
Protein A Sepharose beads washing buffer Tris-HCl pH 8.0 20 mM
Sodium chloride (NaCl) 500 mM
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 2 mM
Sodium dodecyl sulfate (SDS) 0.1% (w/v)
IGEPAL-CA630 1% (v/v)
SDS Lysis buffer Sodium dodecyl sulfate (SDS) 1% (w/v)
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 10 mM
Tris-HCl pH 8.1 50 mM
TE buffer Tris-HCl pH 8.0 10 mM
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 1 mM