Name of the buffer
|
Reagent
|
Final concentration
|
Dilution buffer |
Sodium dodecyl sulfate (SDS) |
0.01% (w/v) |
Triton X-100 |
1.1% (v/v) |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
1.2 mM |
Tris-HCl pH 8.1 |
16.7 mM |
Sodium chloride (NaCl) |
167 mM |
DMA solution |
Dimethyl adipimidate (DMA) |
10 mM |
Phosphate-buffered saline (PBS) |
1x |
Elution buffer |
Sodium dodecyl sulfate (SDS) |
1% (w/v) |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
10 mM |
Tris-HCl pH 8.0 |
50 mM |
High salt buffer |
Sodium dodecyl sulfate (SDS) |
0.1% (w/v) |
Triton X-100 |
1% (v/v) |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
2 mM |
Tris-HCl pH 8.1 |
20 mM |
Sodium chloride (NaCl) |
500 mM |
IMDM medium |
Iscove's modified Dulbecco's medium (IMDM) |
1x |
Fetal bovine serum (FBS) |
2% (v/v) |
Penicillin/Streptomycin |
1x |
Peptone primatone |
0.3 mg/mL |
Insulin solution human |
4.8 mg/mL |
Minimum essential medium non-essential amino acids (MEM NEAA) |
1x |
LiCl salt buffer |
Lithium chloride (LiCl) |
0.25 M |
IGEPAL-CA630 |
1% (v/v) |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
1 mM |
Tris-HCl pH 8.1 |
10 mM |
Low salt buffer |
Sodium dodecyl sulfate (SDS) |
0.1% (w/v) |
Triton X-100 |
1% (v/v) |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
2 mM |
Tris-HCl pH 8.1 |
20 mM |
Sodium chloride (NaCl) |
150 mM |
Protein A Sepharose beads washing buffer |
Tris-HCl pH 8.0 |
20 mM |
Sodium chloride (NaCl) |
500 mM |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
2 mM |
Sodium dodecyl sulfate (SDS) |
0.1% (w/v) |
IGEPAL-CA630 |
1% (v/v) |
SDS Lysis buffer |
Sodium dodecyl sulfate (SDS) |
1% (w/v) |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
10 mM |
Tris-HCl pH 8.1 |
50 mM |
TE buffer |
Tris-HCl pH 8.0 |
10 mM |
Ethylenediaminetetraacetic acid (EDTA) pH 8.0 |
1 mM |