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. 2017 Jun 17;(124):55907. doi: 10.3791/55907
Antibody Supplier Amount of antibody/immunoprecipitation Amount of cells/immunoprecipitation Washing conditions
H3 Abcam (ab1791) 2.5 mg 5 x 106 Once in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer
H3K4me1 Abcam (ab8895) 2.5 mg 5 x 106 Once in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer
H3K4me3 Diagenode (pAb-003-050) 2.5 mg 5 x 106 Once in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer
H3K27ac Diagenode (pAb-174-050) 2.5 mg 5 x 106 Oce in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer
IgG Diagenode (C15410206) Variable* Variable* Variable*
* In the case of the IgG control, the amount of both antibody and cells, as well as the washing steps, have to mirror the conditions of the other immunoprecipitations.