Antibody
|
Supplier
|
Amount of antibody/immunoprecipitation
|
Amount of cells/immunoprecipitation
|
Washing conditions
|
H3 |
Abcam (ab1791) |
2.5 mg |
5 x 106
|
Once in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer |
H3K4me1 |
Abcam (ab8895) |
2.5 mg |
5 x 106
|
Once in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer |
H3K4me3 |
Diagenode (pAb-003-050) |
2.5 mg |
5 x 106
|
Once in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer |
H3K27ac |
Diagenode (pAb-174-050) |
2.5 mg |
5 x 106
|
Oce in low salt buffer, twice in High salt buffer, twice in LiCl salt buffer and three times in TE buffer |
IgG |
Diagenode (C15410206) |
Variable* |
Variable* |
Variable* |
* In the case of the IgG control, the amount of both antibody and cells, as well as the washing steps, have to mirror the conditions of the other immunoprecipitations. |