Figure 8.

Representative In Situ Zymography with Aortic Sections Demonstrate a Decrease of MMP Activity in the Aortic Media of AP-1 cODN (cAP1)-Pretreated Grafts in Comparison to Non-Treated (Control) or AP-1 mODN (mAP1) Grafts

Either a photographic emulsion containing gelatin (A) or a fluorescin-labeled gelatin substrate (B) is brought into contact with the tissue sections. After incubation, MMP enzymatic activity is revealed as white spots on a dark background (A) or as bright green fluorescence (B), respectively. (C) Statistical summary of the mean brightness value analysis of each group (cAP1 versus control, *p < 0.0001, n = 5; cAP1 versus mAP1, #p < 0.0001, n = 4) of the fluorescin-gelatin zymography.