Fig. 7.

Effect of Mito-TEMPO treatment on the survival rate, and functional and morphological damage to the livers of Idh2^+/+ and Idh2^-/- mice after HIR. Mice were treated with mito-TEMPO (mito-T), twice, 17 h and 1 h before either hepatic ischemia or sham-operation and livers were harvested 5 h after surgery. (A) Cumulative survival rates were determined until 5 h after HIR. Data are expressed as the cumulated survival rates (B, C) Five hours after surgery, plasma concentrations of AST (B) and ALT (C) were determined. (D, E) Liver sections were stained with PAS reagent and the damaged areas were evaluated. The results are expressed as the mean ± SEM. *, p < 0.05 vs. respective sham-operated mice. †, p < 0.05 vs. respective vehicle-I/R. #, p < 0.05. NS, no significance.