Figure 2.

NK cells survive for 21 days after adoptive transfer to autologous humanized mice. Ex vivo expanded CB-NK cells were adoptively transferred into either autologous humanized NRG or control mice through i.v. injection. Blood was collected via facial bleeds at the respective time points. PBMCs were stained with anti-mouse hCD45, hCD3 and hCD56. The hCD45+ population was analyzed using a cross gate on hCD3 and hCD56. NK cells were defined as hCD56⁺hCD3⁻ events. (A) Representative flow cytometry plots of human lymphocyte populations at days 0, 1, 7, 14 and 21 days post cell transfer. Groups included humanized mice that received an autologous NK cell transfer, NRG mice that received the same NK cells and humanized mice that did not receive any cells as controls for baseline populations. (B) The percentage of hCD56+ hCD3− cells in humanized and control NRG mice after adoptive transfer was analyzed. The experiment was conducted twice, using 4 mice per group each time. Results were analyzed via two-way ANOVA with Bonferroni multiple comparisons.