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. 2017 Sep 21;7:12048. doi: 10.1038/s41598-017-12162-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optogeneticaly induced upregulation of the Cox-2 regulate the downstream PGE₂ effector molecule in PACs expressing mammalian (HEK-293T) cells. The PGE₂ concentration was determined in the supernatant of HEK-293T cells transfected with empty vector (pA3M) or PACs (NgPAC1, NgPAC2, NgPAC3 and bPAC) in dark and after treated with blue light (460 nm). Data from dark-adapted cells are displayed as solid bar; data from illuminated cells are shown as filled bar. ***p < 0.0005, **p < 0.005, measured by student’s t test between dark and light conditions. Error bars represents the mean of S.E.M, n = 4 experiments in duplicate.