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. 2017 Sep 21;7:12048. doi: 10.1038/s41598-017-12162-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of blue light induced activation of PACs on D. discoideum development at 20 h. Phenotype of (a) Ax2/act15:: GFP, (b) Ax2/act15:: GFP-NgPAC1, (c) Ax2/act15:: GFP-bPAC cells at 20 h of development stage, when developed on KK₂ non-nutrient agarat a density of 1 × 10⁶ cells/cm². The plates were kept in the dark for first 10 h (black bar) after which the plates were exposed to blue light for next 10 h (blue bar). Developmental stages at 20 h were scored and photographed at the time as indicated by open arrow head.