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. 2017 Sep 21;7:12084. doi: 10.1038/s41598-017-12360-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ex vivo expansion of G-CSF mobilized CD34⁺ HSPCs treated for 5 days with HDACIs. (a) Representative flow-cytometry analysis of CD34 and CD90 expression after 5 d of ex vivo HDACI treatment. (b) Absolute cell numbers of CD34⁺ cells. (c) Absolute cell numbers of CD34⁺CD90⁺ cells. Both CD34⁺ and CD34⁺CD90⁺ cells were significantly increased after 5 days treatment with VPA (1 mM), resminostat (1.5 µM) or entinostat (1.5 µM) compared to controls (PBS and DMSO; n = 5). Data are shown as mean ± SD, ***p < 0.001.