Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 21;7:12084. doi: 10.1038/s41598-017-12360-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ex vivo VPA-expanded CD34⁺ cells exhibit increased adhesion to MSCs. (a) Freshly isolated CD34⁺ cells were seeded onto a confluent MSC layer. Representative images after 5 days of co-culture with MSCs showed an increase in the number of VPA-treated adherent cells (right) compared to control cells (left). Images were taken after sufficient washing with PBS. (Scale bar: 250 µm). (b) In the adhesion assay, a significantly higher number of VPA expanded cells were attached to MSCs compared to control (n = 4). (c) Schematic representation of atomic force microscopy-based single-cell force spectroscopy used to measure adhesive strength of VPA treated and control cells. (d) Plot showing detachment force measurement after 5 days of VPA or PBS (control) treatment. Adhesive strength of VPA treated cells was 2–3 fold higher than control (n = 3). Data are mean ± SD, ***p < 0.001.