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. 2017 Sep 21;7:12064. doi: 10.1038/s41598-017-10924-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cannabidiol treatment on chronic-binge ethanol-induced dysregulation of metabolic genes in the liver. Realtime PCR analyses of genes involved in liver fatty acid biosynthetic (FASN, MLYCD and ACC-1) (A) and oxidation pathways (ADIPOR-1 and MCAD) (B), mitochondrial pathway (mCPT-1) (C) and transcription factor PPAR α (D). Values represent means ± SEM (n = 4–7). *P < 0.05 vs. Pair-fed group, ^# P < 0.05 vs. EtOH group determined by One-way ANOVA, followed by Tukey’s post-hoc test. (E) Western blot analyses of FASN, ACC1, PPARα with loading control β-actin. (F) Quantification of western blot data for FASN, ACC1 and PPARα. Values represent means ± SEM (n = 4/group). *P < 0.05 vs. Pair-fed group, ^# P < 0.05 vs. EtOH group determined by One-way ANOVA, followed by Tukey’s post-hoc test. The full-length blots are included in the Supplemental information file.