Figure 5.

Effect of cannabidiol treatment on the hepatic neutrophil function in mice and in human neutrophils. (A) Neutrophils were isolated from livers of mice after chronic binge alcohol diet and incubated with PMA in vitro. The production of ROS was then measured (DHR mean intensity). Values represent means ± SEM (n = 3/group). *P < 0.05 vs. EtOH group determined by Student’s t-test. (B) Representative dot blot analyses of human granulocytes from blood using forward and side scatter followed by CD11b and CD66b staining. Double positive cells were gated for ROS measurement. (C) Representative histogram of ROS intensity (FL1) of human neutrophils treated with CBD (1 µM) and/or CB₂ antagonist SR144528 (1 µM) followed by PMA treatment (100 µg/ml) in vitro. (D,E) Quantitative analyses of DHR mean intensity expressed as fold change (compared to vehicle). Values represent means ± SEM (n = 4/group). *P < 0.05 vs. vehicle group, ^# P < 0.05 vs. PMA group determined by One-way ANOVA, followed by Tukey’s post-hoc test.