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. 2017 Sep 21;7:12064. doi: 10.1038/s41598-017-10924-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cannabidiol on ethanol-induced liver inflammation. Hepatic mRNA levels of MIP-2 (A), MCP-1 (B), TNF-α (C), IL-1β (D), and adhesion molecules E selectin (E) were analyzed by real-time PCR. The results are expressed as fold increases relative to the pair-fed groups. Values represent means ± SEM (n = 5–7). *P < 0.05 vs. Pair-fed group, ^# P < 0.05 vs. EtOH group determined by One-way ANOVA, followed by Tukey’s post-hoc test. (F) Quantitative ELISA of cytokine TNF-α (F) and E selectin (G) in liver tissue lysates. Values represent means ± SEM (n = 4/group). *P < 0.05 vs. Pair-fed group, ^# P < 0.05 vs. EtOH group determined by One-way ANOVA, followed by Tukey’s post-hoc test.