Fig. 1.
The expression of GFP-tagged MetRSL274G upon ‘Stop signal’ excision. a Schematic representation map of the allele after CMV-Cre mediated ‘Stop signal’ excision (MetRSL274G mice). b QT PCR demonstrates that transgene expression is indeed induced upon Cre-LoxP mediated excision of the ‘STOP’ signal. *P < 0.05, Wilcoxon rank sum test. s.e.m., n = 3 for each genotype. c Cells were isolated from indicated genotypes and cultured in F10, 20% FBS for 24 h after which the cells were imaged on bright field and for GFP fluorescence. d Muscles from three separate mice were dissected, cryo-sectioned and imaged on bright field and for GFP fluorescence. 3–4 experimental replicates (sections) were assayed for each animal. Representative images are shown. Scale bar, 100 μm